After their initial victory over the Dark Queen, the Battletoads thought they could relax. But peace is short-lived as the Earth's defenses are suddenly wiped out and a massive spaceship, the Colossus, appears from behind the moon. The Dark Queen is back, and this time she's teamed up with the Shadow Warriors from the Double Dragon series to conquer the galaxy! To tackle this new threat, the Battletoads join forces with the legendary Lee brothers, Billy and Jimmy. Together, this fearless team of five sets out on an epic mission to take down the Dark Queen and her allies, saving the universe from their evil plans.